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PerkinElmer

Molecular Karyotyping

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Fast, precise, and cost-effective targeted molecular karyotyping

BACs are Bacterial Artificial Chromosomes, large cloned sequences of human DNA typically 170,000 bases long. BAC probes have long been used for FISH. The BACs-on-Beads? technology uses probes generated from selected BACs immobilized onto Luminex? xMAP? encoded beads. The resulting bead sets are used to assay chromosomal gains and losses from minute sample amounts with high throughput. BACs-on-Beads analysis comprises hybridization of DNA sample sequences to BAC-derived probes representing the identified target regions. Samples are enzymatically labeled with biotin, and after hybridization in a 96-well plate a fluorescent streptavidin-phycoerythrin reporter is bound to the biotin label. Male and female reference DNA is also hybridized and the fluorescence ratio between each sample and reference DNA are compared to provide a clear display of any copy number changes in the targeted regions. Sample labeling is done enzymatically, which provides significant amplification of samples down to 100-200 ng.

For research use only. Not for use in diagnostic procedures.

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  • AutoDELFIA-B098-201-PAPP-A

    Constitutional BoBs??Kit

    The Constitutional BoBs? kit product places the benefits of the BoBs technology at the disposal of researchers needing a fast and convenient way to investigate copy number changes of five chromosomes, while simultaneously detecting aberrations in 9 specially selected microdeletion regions.
  • AutoDELFIA-B098-201-PAPP-A

    KaryoLite BoBs? Kit

    KaryoLite BoBs? kit has been developed to detect arm-specific aneuploidies in all 24 chromosomes in a single assay for research use only. The product covers p and q arms of all chromosomes 1-22, X and Y (q arms in acrocentric chromosomes).
  • AutoDELFIA-B098-201-PAPP-A

    CGX Oligo Arrays (outside USA)

    CGX Oligo Arrays are microarrays specifically designed, developed and verified by PerkinElmer for the detection of small genetic aberrations associated with learning disability and dysmorphic features in research applications.
  • AutoDELFIA-B098-201-PAPP-A

    CGX Onco Arrays (outside USA) Onco (2 pack)

    CGX? Onco Arrays are research microarrays specifically designed, developed and verified by Signature Genomics for the detection of small genetic aberrations associated with hematological disorders such as leukemias and lymphomas, as well as genes associated with solid tumors.
  • AutoDELFIA-B098-201-PAPP-A

    CGX Onco Arrays (outside USA) Onco (4 pack)

    CGX? Onco Arrays are microarrays specifically designed, developed and verified by Signature Genomics for the detection of small genetic aberrations associated with hematological disorders such as leukemias and lymphomas, as well as genes associated with solid tumors.
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