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Application Note

Estrogen Monitoring in River Water by LC/MS/MS with Online SPE

Introduction

Endocrine disrupting chemicals (EDCs) have been found to have harmful effects on the health of both humans and wildlife. Of these EDCs, endogenous estrogens, such as estriol, 17?-estriadiol, and estrone are of particular interest due to their potency/physiological activity even at very low levels. These hormones are particularly challenging to monitor, as concentrations are typically expected to be in the low ng/mL (ppb) to low pg/mL (ppt) range.

To improve sensitivity, this work utilizes the addition of on-line solid phase extraction (SPE) coupled to an LC/MS/MS system for sample enrichment and quantitation. This approach allows for significant/efficient analyte concentration, obviating the need for elaborate and time-consuming sample preparation procedures. Due to enrichment, compared to other methods, large sample volumes (= ? liter) are no longer needed to reach part per trillion levels for estrogen monitoring in river water.

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